Flag Peptide – 5mg & 10mg Vial

Flag Peptide, often referred to as FLAG-tag Peptide, is a short hydrophilic amino acid sequence (DYKDDDDK) widely used in biotechnology, molecular biology, and immunological studies. It is primarily employed as an epitope tag in recombinant protein expression and purification systems, owing to its high specificity and compatibility with monoclonal anti-FLAG antibodies.

Flag peptide allows for efficient protein detection, quantification, and isolation from complex biological samples. Available in both 5mg and 10mg lyophilized vials, our product is synthesized under stringent quality conditions, HPLC-purified, and validated for research use.

📦 Product Specifications

🔬 Scientific Applications

Flag peptide is predominantly used in experimental research to:

1. Tag recombinant proteins for easier purification via affinity chromatography.

2. Detect proteins in Western blot, ELISA, flow cytometry, and immunoprecipitation using anti-FLAG antibodies.

3. Facilitate protein–protein interaction assays.

4. Track intracellular protein localization using confocal microscopy.

5. Quantify expression levels in transfection experiments.

Its short, hydrophilic nature minimizes interference with the function of the target protein, making it a preferred epitope tag in both in vitro and in vivo systems.

🧪 Reconstitution & Handling

• Reconstitution: Add sterile distilled water or buffer to reach the desired concentration. Mix gently until fully dissolved.

• Suggested Diluent: 10mM Tris buffer or PBS (pH 7.4).

• Handling: Use aseptic techniques; avoid repeated freeze-thaw cycles for best results.

• Storage post-reconstitution: Store aliquots at -20°C for up to 3 months.

⚠️ Important Usage Notice

This product is intended strictly for laboratory research use only. It is not approved for human consumption, medical applications, clinical diagnosis, or veterinary use. All buyers must be qualified researchers or affiliated with authorized research institutions.

✅ Why Choose Our FLAG Peptide?

• ✅ HPLC-Purified and Mass-Spec Verified

• ✅ 98%+ Purity for reliable reproducibility

• ✅ Fast, discreet shipping across the USA

• ✅ Backed by Certificate of Analysis (CoA)

• ✅ Available in both 5mg & 10mg research-use vials

• ✅ Secure packaging & batch traceability

• ✅ Free consultation for large-volume research buyers

🌐 Compliance & Quality Control

Each vial undergoes rigorous quality assurance including:

• HPLC Purity Check

• Mass Spectrometry Validation

• Microbial Limit Testing (when applicable)

• Batch-specific CoA available upon request

Manufactured in ISO-compliant peptide synthesis labs under cGMP-like conditions for research chemical handling.